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The Role of Histochemical and Immunohistochemical Methods for The Detection of Helicobacter Pylori in Chronic Gastritis

Iraqi Postgraduate Medical Journal, 2022, Volume 21, Issue 1, Pages 76-81
10.52573/ipmj.2021.174074

Abstract

BACKGROUND:
Helicobacter pylori represents one of the most common pathogen worldwide. Infection with it can cause medical problem and establishe as etiologic factor in the development of gastritis, So                            the accurate detection is important for proper patient management.
OBJECTIVE:  
This study was conducted to assess the detection of H pylori by Hematoxylin & Eosin, special stain (Modified Giemsa stain) and immune stain in gastritis biopsies and to assess the sensitivity and specificity of each method use in detection.
MATERIAL AND METHODS:
A retrospective and prospective (case series) study, from October 2019 to October 2020, 100 gastric biopsies with gastritis referred to histopathology department, in AL-Khansaa Teaching Hospital and some private labs in Mosul city, were examined.
All biopsy specimens examined histologically to assess H. pyloriinfection, by Hematoxylin & Eosin , modified Giemsa and immunohistochemical stains.
RESULTS:
Patients age range 17 to 78 years with a mean+­­- SD of 44.5+6.11   years. H. pylori positive with H&E /MGS in 79% of cases, while with IHC in 88% of cases. The sensitivity and specificity of the H&E /MGS comparing with the IHC stain were 90% and 100% respectively.
CONCLUSION:
We have concluded from this research that H& E is a reliable way to detect H. Pylori infection. As well as we recommend the usage of IHC in special cases.
 
 
Keywords:

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